Fig. 2.
Western blots for nerve growth factor (NGF) immunoreactivity in skin from sham operated and incised rats. (A) Example Western blot using NGF antibody against mature rat NGF (approximately 16 kd) and skin samples from sham-operated and incised rat hind paw skin from six rats. (B) Example of Western blot using a detailed time course after plantar incision. (Top) NGF immunoreactive bands. (Bottom) Immunoreactive actin control bands. (C) Summary of relative changes in NGF protein expression after plantar incision. Data are expressed as mean ± SD of ratio of incised skin to sham controls. POD = postoperative day. *P< 0.05, **P< 0.01versussham. n = 6 per group.