Fig. 4. Wash after azi-etomidate modification: reversible  vs. irreversible effects. (  A and  B ) Currents from a single HEK293 cell both before treatment (  A : premodification) and during wash after treatment with 1 μm azi-etomidate + 10 μm GABA + 365 nm light. Currents were elicited with 1 mm γ-aminobutyric acid (GABA).  Bars over traces indicate GABA application, and traces are labeled with wash time in minutes (the 6-min washout trace is unlabeled for clarity). (  C ) Peak current amplitude from Panels  A and  B is plotted  vs. wash time. For this cell, recovery is 90% complete in 6 min and I^post/I^pre= 0.85. (  D ) Deactivation τ^w(weighted average time constant) is plotted  vs. wash time. Deactivation continues to slow during wash. (  E ) Maximal currents after treatment and wash normalized to pretreatment currents  vs. azi-etomidate. Cells were treated with azi-etomidate + 10 μm GABA + 365 nm light for 5 min and washed for at least 10 min. Average I^post/I^pre± SD results are shown from at least three cells or patches at each azi-etomidate concentration. (  F ) Washout time  vs. azi-etomidate. Cells were treated as described for Panel  E and time to 90% recovery of maximal amplitude (I¹⁰⁰⁰) or deactivation weighted time constant (τ^w) was recorded. Whereas the averaged data show a trend toward slower washout after treatment with higher azi-etomidate concentrations, there were no significant differences among groups.