Fig. 5. Electron microscopic views of the hearts stained to reveal the glycocalyx. ( A and B ) Control experiment after 20 min of warm ischemia and reperfusion. The endothelial glycocalyx is nearly completely degraded, and a significant formation of edema is visualized. A is an overview, and B is a close-up view of the degraded glycocalyx. ( C and D ) After treatment with hydrocortisone and 20 min of warm ischemia and reperfusion. Endothelial glycocalyx is mostly intact, and less edema formation can be seen. C is an overview, and D is a close-up view of the intact glycocalyx. ( E and F ) Heart perfused for 35 min without ischemia. E is an overview, and F is a close-up view of the intact glycocalyx.