Figure 2. (A) Average currents (8–15 single recordings) from one outside-out patch, elicited by 10 sup -4 M acetylcholine alone or combined with sevoflurane as indicated. Holding potential -30 mV. The peak currents were -80 pA, -75 pA, -56 pA, -14 pA, and -82 pA at 10 sup -4 M acetylcholine, 4.9 x 10 sup -5 M, 4.9 x 10 sup -4 M, 4.9 x 10 sup -3 M sevoflurane, and the control, respectively. The insets with an expanded time scale show the reduction of the peak amplitudes in more detail. (B) Semilogarithmic plots of the current decay (the current was normalized before it was plotted on a log scale). The decay was fitted by one or two exponentials with time constants as indicated. Note the different time scales.