Fig. 3.
Use-dependent block of Na+ currents by bulleyaconitine A (BLA) and by aconitine. (A) Superimposed traces of Na+ currents were recorded in the presence of 10 μm BLA with 1,000 repetitive pulses (+50 mV for 4 ms) applied at 2 Hz. The cell was perfused with 10 μm BLA approximately 5 min before repetitive pulses. Current traces are labeled with the number of corresponding pulses, ranging from 1 to 1,000. (B) Superimposed traces of Na+ currents were recorded in the presence of 10 μm aconitine with 1,000 repetitive pulses. The conditions were the same as described inA.