Fig. 1. Fluoro-Jade positive staining in the mouse brain at the level of the lateral ventricle (LV) and the olfactory bulb upper part (OB up). Degenerating neurons 24 h after exposure to ketamine (25 mg/kg) plus propofol (10 mg/kg) in the stria terminalis (St) ( A and C ) and OB up ( B and D ). Slides (10 μm thick) were semiquantitatively analyzed for Fluoro-Jade positive (degenerative) cells, using Adobe PhotoShop 5.5 (Adobe Systems Incorporated, San Jose, CA) and Scion Image (Frederick, MD) software programs. The figure illustrates the anatomical regions and the area of degeneration measured with Scion Image software.