Fig. 5. Effects of localized inflammation of the dorsal root ganglion (LID) on the tetrodotoxin (TTX)-resistant Na⁺current. TTX-resistant current was isolated using a holding potential of −50 mV, which largely inactivates the TTX-sensitive current. (  A ) Peak TTX-resistant current density  versus voltage in isolectin B4 (IB4)-positive cells. * Individual voltages for which LID significantly different from control (CON) (two-way repeated-measures analysis of variance). (  B ) Peak TTX-resistant current density  versus voltage in IB4-negative cells. None of the differences were significant. (  C ) Voltage dependence of activation, obtained by normalizing the data from each cell to the value at 0 mV. (  D ) Steady state inactivation  versus prepulse voltage. Currents were measured during a test pulse to −20 mV, and the TTX-sensitive and -resistant currents were separated as described in the Materials and Methods. In  C and  D , data from IB4-positive and IB4-negative cells are combined because there were no differences between the subgroups observed.