Fig. 3. Immunoblot of secreted ExoU, ExoS, and PcrV from Pseudomonas aeruginosa strains. ( A ) Top line numbers indicates number of strains, and bottom line numbers indicate the blind bronchoalveolar lavage plasminogen activator inhibitor-1 (PAI-1) concentrations. Top line immunoblot indicates ExoU secretion, with a KD of approximately 70; middle line immunoblot indicates ExoS secretion, with a KD of approximately 50; and bottom line immunoblot indicates PcrV secretion, with a KD of approximately 37. PA01 served as the control P. aeruginosa strain. ( B ) Correlation between PAI-1 concentration and ExoS secretion. Spearman analysis showed a significant correlation between PAI-1 and ExoS ( P = 0.03). ( C ) Correlation between PAI-1 concentration and ExoU secretion. Spearman analysis showed a significant correlation between PAI-1 and ExoU ( P = 0.04).