Fig. 4. Interactions between volatile anesthetics and cyclic guanosine 3′,5′-cyclic monophosphate (cGMP). As with fig. 3 , store-operated Ca2+entry was triggered three times (with intervening washes). However, 100 μm 8-Br-cGMP was used instead of dibutryl cAMP (dbcAMP). Store-operated Ca2+entry was also significantly decreased by 8-Br-cGMP. In the presence of halothane (Hal), isoflurane (Iso), or sevoflurane (Sevo), store-operated Ca2+entry inhibition was much greater than that with anesthetic or 8-Br-cGMP alone. * Significant difference from control; # significant difference from both anesthetic and 8-Br-cGMP ( P < 0.05). CPA = cyclopiazonic acid.