Fig. 9. Carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) does not affect 4-chloro- m -cresol (4-CmC)–induced intracellular Ca2+release. Dakiki cells in suspension in Ca2+-free Hanks balanced salt solution. ( A ) FCCP, 2 μm, applied at 30 s; 1 mm 4-CmC at 120 s. Tracing is from a single experiment. ( B ) Y-axis = peak minus baseline just before addition of drug. Bars 1 and 2 : 4-CmC–induced intracellular Ca2+signal before or after application of FCCP; means not significantly different. n = 13 and 15, respectively. Bars 3 and 4 : FCCP-induced intracellular Ca2+signal before or after 1 mm 4-CmC; means significantly different ( P = 0.0085). n = 25 and 11, respectively.