Fig. 5. Effect of azumolene on 4-chloro- m -cresol (4-CmC)–induced Ca2+i. Dakiki cells in 0 Ca2+Hanks balanced salt solution plated for confocal microscopy. ( A ) Upper trace : 1.5 mm 4-CmC applied at 150 s and washed off at 550 s. Lower trace : 100 μm azumolene throughout; 1.5 mm 4-CmC applied at 150 s and washed off at 450 s. ( B ) Upper trace : 1.5 mm 4-CmC applied at 80 s and washed off at 380 s. Lower trace : 400 μm azumolene throughout; 1.5 mm 4-CmC applied at 80 s and washed off at 300 s. Each trace is the average of 43–59 single cell records taken from a single field for that condition.