Fig. 5. Western blot analysis of ( A ) ryanodine receptor (RyR2) protein, ( B ) sarcoplasmic reticular Ca2+-pump adenosine triphosphatase (SERCA2a) protein, and ( C ) protein content in homogenates of myocardial tissue from control and ischemic guinea pig hearts. Blots show quantity of RyR2, SERCA2a, and phospholamban protein. Bands are quantitative immunoblots of representative samples from hearts of different groups run in the same gel. Group results furnish values (mean ± SEM) in the treatment groups are expressed as percentage of the control (CTL) group. *P < 0.05 CTL versus all other ischemic groups. APC = anesthetic preconditioning; ISC = ischemia; KBR = KB-R7943; SEA = SEA0400.
