Fig. 7. ( A ) Representative Western blots of slice homogenates with antibodies to phosphorylated ERK (P-p42/44), the survival-associated protein Akt (protein kinase B, band is molecular weight approximately 65 kd), and a control protein (β-actin) in control cultures, and in cultures treated with oxygen and glucose deprivation (OGD), 1% isoflurane (Isof), or a combination of isoflurane and OGD (Isof/OGD). B and C show the intensity of immunostaining in Western blots for phospho-ERK and Akt relative to the band intensity of the normoxic controls.