Fig. 2. Treatment protocols. Anesthetic postconditioning (PostC) = isoflurane administered for 15 min (1.5 minimum alveolar concentration [MAC]) right at the onset of reperfusion after 40 min of test ischemia; CTRL = time-matched perfusion of remodeled hearts; ISCH = unprotected remodeled hearts exposed to 40 min of ischemia alone; LY = LY294002. LY294002 dissolved in dimethyl sulfoxide (DMSO; < 0.1%) was coadministered with isoflurane during early reperfusion. Reperfusion injury salvage kinases (RISKs) were assayed after 15 min of reperfusion, and infarct size was determined after 90 min of reperfusion. Five hearts were used in each group.