Fig. 3. Capillary protein leakage measured by Evans blue dye extravasation ( A ) and formation of lung edema measured by wet/dry lung weight ratios ( B ) were determined in mice receiving saline (Sal), lipopolysaccharide (LPS), or isoflurane (Iso), 1 or 12 h before lipopolysaccharide. Endotoxin aerosol induced a significant increase in both capillary protein leakage and edema formation, which was reduced by isoflurane pretreatment. Data are mean ± SD from n = 3 animals in each group. # Significant change between control and endotoxin-treated mice. * Significant difference between animals with and without isoflurane treatment.