Fig. 2. Effects of enflurane on spontaneous inhibitory postsynaptic currents (IPSCs) recorded from neocortical neurons of wild-type and β3(N265M) mutant mice. ( A – D ) Data obtained from wild-type mice. ( A ) Continuous recordings (12 s) under control conditions ( upper three traces ) and in the presence of 0.6 mm enflurane ( bottom three traces ). ( B ) Mean IPSC calculated from synaptic events occurring during a 180-s recording under control conditions. ( C ) In the presence of 0.3 mm enflurane (approximately 0.5 minimum alveolar concentration [MAC]), the amplitude of the averaged IPSC is only slightly reduced, whereas the decay time is prolonged. ( D ) These dual actions of enflurane are further enhanced at 0.6 mm enflurane (approximately 1 MAC). ( E – H ) Actions of enflurane on IPSCs recorded from β3(N265M) mutant mice neurons. ( E ) Representative trace (12 s) in the absence ( upper three traces ) and in the presence of 0.6 mm enflurane ( bottom three traces ). ( F ) Mean IPSC obtained from a 180-s recording under control conditions. ( G ) In the presence of 0.3 mm enflurane, amplitude and current decay of the averaged IPSC are almost unchanged. ( H ) At 0.6 mm enflurane (approximately 1 MAC), the amplitude of the mean IPSC remains unchanged, whereas the decay time is only moderately prolonged in the β3(N265M) mutant neuron.