Fig. 1. Midazolam inhibits dorsal root evoked polysynaptic excitatory postsynaptic currents (EPSCs). ( A and B ) Averaged traces of six consecutive Aδ-fiber ( A ) and C-fiber ( B ) intensity stimulation evoked polysynaptic EPSCs before and during the application of midazolam (1 μm) and after washout. These were recorded in two different neurons. ( C ) Amplitude and integrated area of Aδ- and C-fiber intensity stimulation evoked polysynaptic EPSCs in the presence of midazolam, relative to those in the control (n = 10 and n = 5, respectively). ( D ) Dose-dependent inhibition of Aδ-fiber intensity stimulation evoked polysynaptic EPSCs by midazolam. For each dose, n > 6. ( E ) Inhibitory action of Aδ-fiber evoked polysynaptic EPSCs by midazolam (1 μm) was not observed in the presence of flumazenil (5 μm). Vertical bars show SDs. * P < 0.01.