Fig. 2. Scheme of treatment protocols. Anesthetic postconditioning (PostC): after 40 min of test ischemia, isoflurane was administered for 15 min (1.5 minimum alveolar concentration [MAC]) immediately at the onset of reperfusion. Atractyloside and LY294002 were coadministered with isoflurane during early reperfusion. In each group, five hearts were used. ATR = atractyloside (10 μm in < 0.1% DMSO); CTRL = time-matched perfusion; DMSO = dimethyl sulfoxide; GSK3β= glycogen synthase kinase 3β; ISCH = unprotected hearts exposed to 40 min of ischemia only; LY = LY294002 (15 μm in < 0.1% DMSO [vehicle]); NAD+= nicotinamide adenine dinucleotide; PKB = protein kinase B.