Fig. 5. Representative immunohistochemistry photomicrographs, corresponding histograms, and representative Western blots depicting phospho-Akt ( A ) and Bcl-2 ( B ) expression in neonatal rat ventricular myocytes under control (CON) conditions, after exposure to hypoxia (HYPOX), and after pretreatment with isoflurane in the presence (ISO + HYPOX) or absence (ISO) of hypoxia. * Significantly ( P < 0.05) different from CON; †significantly ( P < 0.05) different from HYPOX, n = 4. Western blotting was repeated at least twice, with similar results as in the blots shown in the figures.