Fig. 4. The effect of activated neutrophils and isoflurane on cytochrome c release ( A , n = 5, histogram and representative Western blot), the percentage of cells with caspase activity ( B , n = 5), and TUNEL-positive staining ( C , n = 10) in adult rat ventricular myocytes. Control conditions (CON), after exposure to activated canine neutrophils (PMN), and after pretreatment with isoflurane in the presence (ISO + PMN) or absence (ISO) of exposure to activated neutrophils. * Significantly ( P < 0.05) different from CON; † significantly ( P < 0.05) different from PMN.