Fig. 2. The effect of hypoxia and isoflurane on cytochrome c translocation ( A , n = 4, histogram and representative Western blot), the percentage of cells demonstrating caspase activity ( B , n = 6), and TUNEL-positive staining ( C , n = 6) in mouse HL-1 cells ( solid bars ) and neonatal rat ventricular myocytes ( hatched bars ). Control (CON) conditions, after exposure to hypoxia (HYPOX), and after pretreatment with isoflurane in the presence (ISO + HYPOX) or absence (ISO) of hypoxia. * Significantly ( P < 0.05) different from CON; † significantly ( P < 0.05) different from HYPOX.