Fig. 3. One representative Western blot experiment of cytosolic fraction showing time course of PKC-ε phosphorylation during desflurane preconditioning. ( Top ) phosphorylated form of PKC-ε; ( center ) total PKC-ε; ( bottom ) actin (as internal standard). The histogram presents densitometric quantification of four experiments as x-fold average light intensity. Data are presented as mean ± SD; * P < 0.05 versus vehicle baseline.