Fig. 4. Detachment of originally adherent leukocytes from salvaged wound blood (dashed ) and venous blood (solid ). Beginning with the lowest shear rate of 50 s−1(indicated by line breaks ) at the end of each adhesion assay, shear rate was doubled stepwise every 20 s up to 1,600 s−1. At the end of each 20-s interval, the percentage of cells that remained bound was determined relative to the number of cells originally adherent at 50 s−1(100%). The common odds ratios for maintaining attached despite increasing shear rates were calculated by using a maximum likelihood approach (see Materials and Methods). The odds ratios of the two groups differed by a factor of 0.2 (99% confidence interval, 0.095–0.41), indicating that the bond strength was largely impaired in the salvaged wound blood group (P < 0.001).
