Fig. 1. (  A ) Protocols for physiologic, histologic and biochemical analyses.  Sampling for WB = sampling whole hearts for Western blotting;  Sampling for ISH = sampling whole hearts for  in situ hybridization;  PKC inhibitor = protein kinase C inhibitor, bisindolylmaleimide. Groups: (  I ) = Time-control; (  II ) = 30-min propofol infusion; (  III ) = 3-min regional ischemia and 90-min reperfusion; (  IV ) = 3-min regional ischemia and 90-min reperfusion including 30-min propofol infusion; (  V ) = a PKC inhibitor and 3-min regional ischemia and 90-min reperfusion including 30-min propofol infusion. (  B ) Representative left ventricular (LV) pressure (P)-volume (V) loops in an ejecting  in situ rat heart during an aortic occlusion.  Solid circles = end-systolic P-Vdata on the enveloping curve;  ESPVR = end-systolic pressure-volume relation;  SV = stroke volume (EDV − ESV);  Ea = effective arterial elastance; ESP/SV. (  C ) a representative ESPVR.  mLVV = midrange LVV;  ESP^mLVV = ESP at mLVV;  PVA^mLVV = systolic pressure-volume area at mLVV. Volume axes indicate normalized absolute LV volume for 1 g LV mass in B and C .