Fig. 11. The effect of sevoflurane and specific p38 inhibitors on Activator Protein-1 ( AP-1 ) DNA binding after phorbol-myristate-acetate ( PMA ) and ionomycin stimulation. CD3 +T lymphocytes were pretreated for 1 h with either the specific p38α/p38β inhibitors SD202190 or SD203580 at the concentrations indicated, incubated with sevoflurane (8 Vol.%) for 23 h, and subsequently stimulated with 15 ng/ml phorbol-myristate-acetate (PMA) and 700 ng/ml ionomycin for 1 h (lanes 2 through 6) or the respective volumes of ppH 2O (lane 1) as vehicle control. Equal amounts of protein from cell extracts were analyzed for AP-1 activity by electrophoretic mobility shift assay. A section of a fluorogram is shown. ◂= position of AP-1 DNA complexes; ○= a nonspecific activity binding to the probe; ◃= unbound oligonucleotide. The data shown are representative of six independent experiments.