Fig. 5. Hematoxylin stained vessel sections at 20× magnification to demonstrate endothelial nuclei. (  A ) Vessel perfused with serum, no air perfusion performed. (  B ) Vessel perfused with serum, air perfusion performed. (  C ) Vessel first perfused with serum plus Perftoran, then air perfusion performed. (  D ) Vessel first perfused with serum plus Pluronic F-127 (PF-127), then air perfusion performed.