Fig. 3. Effect of liposome injection on leukocyte subsets in the inflamed paw: Rats (n = 7/group) were intraplantarly injected with Freund’s complete adjuvant followed by two intraplantar injections of phosphate-buffered saline (PBS; solvent as control), clodronate-containing liposomes, or empty liposomes. Clodronate-containing liposomes induced a significant depletion of ED1+monocytes/macrophages at both time points of inflammation ( A , * P < 0.05, t test; black bar = PBS; white bar = clodronate-containing liposomes; dashed bar = empty liposomes). Clodronate-containing liposomes did not significantly decrease the number of RP-1+polymorphonuclear cells or of CD3+lymphocytes ( B and C , respectively). All data are presented as mean ± SEM. n.s. = not significant.