Fig. 1. Phosphorylation of purified epidermal growth factor receptor (EGFR) in the presence or absence of lidocaine. Purified EGFR was incubated in buffer containing 0.2 mm adenosine triphosphate with or without 100 ng/ml epidermal growth factor (EGF), lidocaine, or both for 5 min at 37°C. Lidocaine reduced EGF-stimulated tyrosine phosphorylation of EGFR. Result displayed in the upper section represents typical immunoblots (IB). pY = antiphosphotyrosine. *P < 0.05, **P < 0.01 versus EGF-stimulated tyrosine phosphorylation without lidocaine; n = 4 for each lane.