Fig. 2. Representative oxygen recordings when vehicle (Con; A  and B ), the complex III inhibitor antimycin A (100 μm; A ), the uncoupler 2,4-dinitrophenol (DNP; 100 μm; A ), or sevoflurane (Sev; 1.3 mm; B ) were added at t = 3 min. A substrate of 10 mm succinate with 10 μm rotenone (S/R) was given at t = 2 min. The reactive oxygen species scavenger manganese(III) tetrakis (4-benzoic acid) porphyrin chloride (TBAP; 10 μm; B ) was given at t = 1 min. Note the differences in state 3 respiration (slope of straight lines ) after addition of 250 μm adenosine diphosphate (ADP) at t = 4 min.