![Fig. 1. Calibration of bis-oxonol fluorescence in human coronary artery smooth muscle cells. (A ) Representative variations of fluorescence intensity. Effects of gramicidin and various extracellular potassium concentrations ([K+]e) on change in relative bis-oxonol fluorescence. Bis-oxonol fluorescence was measured relative to 9 mm [K+]e(percent). At the points indicated (arrows ), the values were evaluated. (B ) Relation between the fluorescence changes in response to increases in K+concentration and membrane potential calculated from the Nernst equation.](https://asa2.silverchair-cdn.com/asa2/content_public/journal/anesthesiology/100/3/10.1097_00000542-200403000-00027/2/27ff1.png?Expires=1716467359&Signature=TBkiajrL7ash3wCwlWpAvv-uvELQ9wPUdxqLwjiGKkZWA4v~6MyD9XLBoxlm9LAUvVzFHLejR0w9LBnPIkIlPQFjkpCCam7BUHtuQdhzcMg96Ynlhfz5GSANWpThSKkjVfrdLkYe9UukuegVnCUr43h8K4aExwuYSaeTXd4~7b464W3~hRRE3KHZEKxcMzB792m9CwjDNl9qVUJwj46b6aHG~czyZnDy-zi9pHZOfcIttScQlmJOnti~ymQVwcURxQmO8BEv6C8XOM~7sbvxIg5kXlUlpOKq1XTM7p6WRQ6~j0E-y702e4AEFl7MZn6LrIJHelNVqbASTy7PbMKcCg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Fig. 1. Calibration of bis-oxonol fluorescence in human coronary artery smooth muscle cells. (A ) Representative variations of fluorescence intensity. Effects of gramicidin and various extracellular potassium concentrations ([K+]e) on change in relative bis-oxonol fluorescence. Bis-oxonol fluorescence was measured relative to 9 mm [K+]e(percent). At the points indicated (arrows ), the values were evaluated. (B ) Relation between the fluorescence changes in response to increases in K+concentration and membrane potential calculated from the Nernst equation.
