Fig. 1. Effect of isoflurane on activation of the sarcolemmal adenosine triphosphate–sensitive potassium channel current (IKATP) by pinacidil at 0.5 mm intracellular adenosine triphosphate. (A ) Example traces of whole cell IKATPmonitored during a 100-ms voltage pulse to 0 mV from the holding potential of −40 mV in the absence (CTL) and presence of 20 μm pinacidil (PIN) and corresponding time course of IKATPelicited by pinacidil (protocol A). Pinacidil-activated current was blocked by 1 μm glyburide (GLB). (B ) Time course of IKATPand example traces of current elicited by pinacidil in the presence of 0.55 mm isoflurane (protocol B). Compared with A , the pinacidil-elicited IKATPwas increased in the presence of isoflurane (ISO + PIN). Glyburide, 1 μm, blocked current elicited by pinacidil. Dotted lines = zero current level.