Fig. 2. Effects of halothane on IK, SK1, and chimera channels. Inside-out patches from oocytes expressing IK, SK1, and chimera channels were excised and examined for halothane response. Currents were measured at a membrane potential of −100 mV in the presence of 10 μm Ca²⁺. Both 100 μM EGTA and 1 mm halothane were applied through piezo-driven fast application system (bars under the traces ). Representative traces of IK (A ), SK1 (B ), chimera A (C ), and chimera B (D ) are shown. Inward currents are shown as downward.