Fig. 3. Time course for c-Fos antibody (Fos Ab) immunoreactivity (from modified Western blots) after direct in vivo  electroporation with 4 μg c-Fos antibody (top ). The relative c-Fos antibody immunoactivity is expressed as a ratio of c-Fos antibody/β actin; protein levels were determined by densitometric scanning of Western blots (bottom ). Note the peak activity on day 2 after electroporation. 4 μg Ab + EP = intrathecal 4 μg c-Fos antibody via  electroporation; 4 μg Ab only = intrathecal 4 μg c-Fos antibody without electroporation; NS Ab + EP = intrathecal 4 μg nonspecific antibody via  electroporation; 0.4 μg Ab + EP = intrathecal 0.4 μg c-Fos antibody via  electroporation; vehicle = saline only. For all groups, n = 3 and values are mean ± SEM. *Different from value for vehicle group (P < 0.05).