Fig. 3. The highest crc  expression in the brains of early pupae. The crc  antisense was used as a probe in A-1 , B-1 , C-1 , D-1 , and E-1  in Canton-S  and A-3 , B-3 , and E-3  in eth^as311 , and the sense probes were used in A-2 , B-2 , C-2 , D-2 , and E-2  in Canton-S . (A ) Larval brain hemispheres (bh ) including the optic lobes (ol ) and ventral ganglion (vg ) in the third instar larvae. (B ) The brain of a 19-h-old pupa. (C ) Eye ganglia (arrows ), subesophageal ganglia (sub ), and esophagus (es ) in the head region of a 48-h-old pupa. (D ) Staining of the brain and optic lobes of a 90-h-old pupa. (E ) In the adult brain, staining was limited to arrow regions (arrows ). For names of organs and developmental stages, refer to Hartenstein. 42 Bar = 50 μm.