Fig. 2. Immunostaining of α-tubulin network in cells from Wistar Kyoto rats (WKY). (A ) Control cells; (B ) pretreatment with nocodazole (5 μm, 30 min) showing the formation of α-tubulin aggregates (arrow ); (C –E ) exposure to 1, 2, and 3% isoflurane, respectively (15 min), revealing the alteration of tubular organization (arrows ); (F ) exposure to 3% isoflurane (15 min), followed by 60 min of wash out, showing the reversibilty of isoflurane effect. Bar = 100 μm.