Fig. 3. Effects of bicuculline on inhibition of glutamate release. Rat cerebrocortical synaptosomes were incubated with 100 μm bicuculline for 200 s before addition of isoflurane or propofol. Data are shown as mean ± SD (n = 3); *P < 0.05, **P < 0.01, ***P < 0.0005 by Student unpaired two-tailed t  test. Propofol (20 μm) did not inhibit KCl-evoked release (data not shown).