Fig. 2. (A–E ) Experimental protocols. Baseline indicates a period of no experimental intervention. In all studies, the fluorescence level was calibrated by dinitrophenol (DNP) and cyanide (CN) at the end of each protocol. 5-HD = 5-hydroxydecanoic acid; ISO = isoflurane; SEV = sevoflurane; DMSO = dimethyl sulfoxide; PRP = propofol; PNT = pentobarbital.