Table 2. Kinetic Parameters of l-[3H]Glutamate and [14C]GABA Uptake
The results from two methods of analysis are shown. Analysis using least-squares curve fit included the determination of maximum velocity (Vmax) in the presence of isoflurane while fixing the Michaelis-Menten constant (Km) to control values (results in parentheses). Statistical significance between control and isoflurane parameters of Vmax(nmol · min−1· mg protein−1) and Km(μm) was tested by paired t tests.