Table 2. Scatchard Analysis of Effect of Ethanol on [3H]DPDPE Binding
Binding of [3H]DPDPE to N18TG2 cells plated in 24-well plates was determined as described in Materials and Methods. Incubations were carried out either for 30 min at 37°C or for 16 h at 4°C. Cells that were not subject to ethanol treatment are designated as control. Results are the mean ± SD of duplicate determinations from three independent experiments; values that represent significant differences from respective controls are indicated.