Table 1. Effect of Pretreatment with Pentobarbital on the Accumulation of HSP27 by Vasopressin in A10 Cells
Cultured cells were pretreated with pentobarbital (0.8 mm) or vehicle. After 48 h, the medium was replaced and the cells were exposed to 0.1 μm vasopressin or vehicle in the presence or absence of pentobarbital (0.8 mm) for 48 h. The concentrations of HSP27 in cell extracts were determined by a specific immunoassay. Each value represents the mean ± SD of triplicate determinations. Similar results were obtained with two other cell preparations.