Table 1. Comparison of the Effects of Intravenous Anesthetics and α-Tocopherol on Glutamate Uptake in Astrocytes Exposed to t-BOOH
Astrocytes were incubated for 3 h in serum-free minimum essential medium (pH 7.3, equilibrated with 5% CO2:95% air; 37°C). They next were incubated for 30 min in HEPES-buffered medium, with 1 mm tert -butyl hydroperoxide (t-BOOH) or without this organic peroxide (control). A 30-min recovery period followed before the initial rate of glutamate uptake was measured in 1-min transport assays. Drugs were administered in ethanol vehicle, either simultaneously with t-BOOH (prophylactic drug treatment) or after the incubation with t-BOOH had ended (delayed drug treatment). In both cases, the drugs were present for the remainder of the experiments.
* P < 0.05 compared with control.
† P < 0.05 compared with t-BOOH–treated cells (n = 3–13 independent experiments with triplicate determinations in each).